The mature fruits from 18 gray jujube producing regions in Southern Xinjiang were used to study the differences of fruit quality indicators in each producing regions and comprehensively evaluate the fruits. Based on the results of previous studies,the eugenic zone of gray jujube in Southern Xinjiang was clarified to provide a reference for improving the quality and efficiency of the jujube industry and scientifically developing the gray jujube industry in Southern Xinjiang.The results showed that there were significant differences in the quality indicators of gray jujube among different production regions. There was a close correlation between each quality. Four common factors were extracted by factor analysis,and their cumulative contribution rate was 82.058%. The contribution rate of each factor was used to calculate the comprehensive score of gray jujube in each producing regions and rank. The 18 producing regions were divided into 3 categories by cluster analysis. The results of factor analysis were basically the same as cluster analysis. The comprehensive fruit quality of gray jujube in the southeast of Tarim Basin was better than that in the northwest. The results of cross-analysis with previous researches showed that the four producing regions including Ruoqiang,Minfeng,Luopu,and Qiemo are not only extremely suitable for the growth and development of gray jujube in terms of climate factors,but the comprehensive quality of gray jujube fruit is better as well. It is indicating that these four producing regions are the best eugenic zones of gray jujube in Southern Xinjiang. The restructuring of the gray jujube eugenic zone in Southern Xinjiang is of guiding significance for developing the jujube industry.