To provide a scientific basis for revealing the differences in metabolites related to flavor quality and improving fruit quality,Liangping pummelo fruits under normal cultivation management and no cultivation management were used to compare and analyze their spectrum of volatile substance and main bitter substances in the fruit with gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The results showed that the total monoterpenoids,total sesquiterpenoids,total non-terpenoids and total volatile compounds in Liangping pummelo fruit under no cultivation management were significantly reduced compared with those in the fruits under normal cultivation management. However,the composition and content percentage of the main volatile compounds remained stable. The content of naringin was significantly increased in the flavedo,albedo,segment membrane and juice sacs of fruits under no cultivation management. The contents of limonin and nomilin were also significantly higher in albedo,segment membrane and juice sacs of fruits under no cultivation management. It is indicated that no cultivation management result in a decrease of the aroma and an increase of bitterness in the fruit of Liangping pomelo. The quality of fresh food was greatly reduced,and consumer acceptance was low. The economic benefits and land output values of orchard under no cultivation management caused by continuing rural-urban migration of middle-aged labor force can be improved to some extent through the help from popular health propaganda of bitter compounds.