The contents of metabolites in the mature kernels of each line from the RIL population constructed by crossing the common wheat Soru#1 with Naxos were determined. The results showed that the contents of 478 known metabolites detected had high broad-sense heritability and varying degrees of content variation. Among these metabolites,the structurally similar metabolite involved in the same metabolic pathway had the similar rule of content distribution. The QTL mapping of contents of metabolites detected resulted in a total of 385 mQTL loci corresponding to 236 metabolites. Five of them are hotspots co-mapped by more than five metabolites,located on the chromosome 5A with 2 hotspots,5B with 1 hotspot,6A with 1 hotspot,and 6B with 1 hotspot,respectively. Three of these hotspots are from Naxos and two are from Soru#1.