Rice as a monocot model plant is one of the few crops that achieve mature tissue culture,but the physiological processes and specific molecular mechanisms in the process of tissue culture are still rarely understood. In this study,the CRISPR/CAS9 gene knockout vector of the CRL1 gene in the rice LBD gene family was constructed and transformed into callus of rice Zhonghua 11. Eight families with missing transgene fragments were obtained. The result of callus induction on the seeds of CRL1 gene knockout material showed that their callus induction was strongly inhibited,similar to the known RNA interference material phenotype of the rice OsIAA10 gene. The results of a subcellular localization of OsIAA10 showed that OsIAA10 was located on the nucleus and the cell membrane. Through the yeast two-hybrid screening library experiment,it was found that OsIAA10 can interact with four transcription factors including OsARF5,OsARF 17,OsARF 21,and OsARF 23 among members of the OsARF family. A yeast one-hybrid point-to-point experiment was performed using the promoterof CRL1 gene as a bait to construct a vector. The results showed that only OsARF5 and OsARF21 can specifically bind to the AuxRE motif in the promoter region of the CRL1 gene and activate the transcription of CRL1 gene. It is indicated that the development pathway of rice lateral roots is directly related to the process of callus induction. There is a conservative mechanism of auxin regulation between rice and dicots,which plays an important role in the process of callus induction in rice seeds.