Megalobrama amblycephala is one of the most important herbivorous economic fish in China.In this study,hybrid F1 individuals of M. amblycephala were used as the mapping population,the highquality and highdensity genetic linkage map was constructed and sexrelated QTLs (quantitative trait loci) were mapped by mining SNP molecular markers through restrictionsite associated DNA sequencing (RAD-seq) technology.The result showed that the linkage map included 10 795 SNPs and 24 linkage groups,with a total length of 2 578.54 cM and an average marker interval of 0.24 cM.Based on the linkage map,Muliti-QTL mapping（MQM） was analyzed to fix QTLs related to gender traits by using Map QTL 6.0 software.The experiential LOD value of 3.0 was taken as the threshold for the existence of QTL tags,and 4 sexrelated QTLS were located in the LG8,LG12,LG15 and LG18 linkage groups.The LOD value of each QTL ranged from 3.18 to 4.17,which explained phenotypic variation ranged from 7.7% to 10.0%.After screening genes near SNP markers in the QTL confidence interval,DCTN2,a key candidate gene,which may be involved in the reproductive process of M. amblycephala,was screened by functional annotation analysis.This research will provide a reference for the development of gendered molecular markers ofM. amblycephala.