Liangyou 6326 as one of the promotion varieties in Hubei Province was used to analyze the effects of water management on grain quality of the ratoon rice. Two different water managements including continuous flooding (CF) and alternate wetting and drying (AWD) were applied in the main season. Milling quality,appearance quality,eating and nutritional quality,and health and safety quality of the ratoon rice was assessed under CF and AWD conditions. Under CF condition ,the brown rice rate,milled rice rate and head rice rate of the main crop was significantly increased by 3.78%,4.45%,and 13.03%,respectively. The brown rice rate,milled rice rate and head rice rate of the ratoon crop under the CF condition were 1.63%,1.26% and 8.03% higher than those under the AWD condition,whereas the chalkiness rate of the ratoon crop was 14.9% lower than that under the AWD condition. No significant difference was found in the eating quality of the ratoon crop under the two different conditions. The Fe content of the ratoon crop was significantly increased by 127.41% under the CF condition compared with that under AWD condition. The change of the Fe uptaking in the ratoon rice plants under the CF condition may result in the accumulation of several heavy metals in grains of the ratoon rice. The ratoon rice has better milling quality,appearance quality and nutritional quality under the CF condition,indicating that the CF condition in main season helps to improve grain quality of the ratoon rice.