The pathogenic fungi causing kiwifruit soft rots were isolated and identified by examining the morphological and microscopic characteristics,combined with the results of pathogenicity testing and rDNA-ITS (internal transcribed spacer) sequencing.The contril effect of carvacrol on kiwifruits soft rot was studied.The main pathogens causing soft rot of kiwifruit after harvest are Botryosphaeria dothidea and Phomopsis sp.,followed by Botrytis cinerea,Fusarium proliferatum and Alternaria sp.. Carvacrol had significant antifungal activity against the eight soft rot pathogens,especially for B.dothidea,with half effective concentration (EC50) of 8.87 μg/L.Simultaneously,carvacrol effectively reduced the incidence of kiwifruit soft rot in a simulated sales environment.The incidence of treatment group was only 50.17% of the control fruit when placed for 8 days,and it had no significant effect on the quality of kiwi fruit (P>0.05).Therefore,carvacrol has potential application value in controlling soft rot of kiwifruit after harvest.