In order to evaluate the feeding value of golden needle mushroom barn,the in vitro rumen fermentation was used to select the best feeding combination,which was further tested in the practice. Firstly,the nutrients components of golden needle mushroom barn and other roughages was measured. Next,six groups of combinations based on previous study were designed and in vitro rumen fermentation was applied to measure pH,NH3 and dry matter degradation to select the best group of combinations. Then,the beef cattle feeding trial was conducted to test the best combination. Sixteen healthy Simonthal hybrid cattle with similar bodyweight and age were selected and randomly assigned to two groups,control group and experimental group,with 8 cows in each group. The control group was fed with the basal TMR and the experimental group was fed with the optimal TMR diet. The pre-trial period was 10 days for adaption,and the experimental period was 80 days. Blood collection and body-weighting were performed at the beginning and end of the trial to evaluate the feeding effects on bodyweight gain,blood physiological parameters and serum biochemical index. The results showed that the nutrient components of golden needle mushroom barn was similar to straw and maize yellow storage; The best feeding combination was maize green storage (50%)+golden needle mushroom barn (30%)+beef grain (10%)+bean curd residue (10%); The best combination significantly increased bodyweight gain (P<0.01),reduced the feed cost and had no detrimental effects on blood physiological parameters and serum biochemical index. To sum up,golden needle mushroom barn could be used as roughage for beef cattle.