A randomized complete block (RCB) design field experiment of Ejing 403 with 2 transplanting densities (283 000 and 376 000 hills/hm2) and 5 fertilizer (N) levels (0,165,195,225 and 255 kg/hm2) was conducted to establish the high-yield cultivation techniques of two-season late japonica and promote “early indica-late japonica” plantation pattern in Hubei Province. Rice yield under different treatments was investigated and the dynamics and characteristics of leaf area index(LAI) growth and aboveground dry matter production in different populations were simulated with Gompertz equation. The results showed that the yield was significantly affected by fertilizer level but not by planting density,indicating that the populations of two-season late japonica has strong self-regulation ability. The appropriate nitrogen level for japonica tested was 225 kg/hm2. The effective panicle number and dry matter production per hectare were significantly increased at appropriate fertilizer level and thus promoted the economic yield. The high matter production depended on the higher accumulation speed and longer fast accumulation period,which were based on the rapid establishment of the appropriate LAI in the populations with high yield. The populations with higher yield had higher LAI growth speed but shorter fast growth period. The suitable population LAI and biomass production at different stages were proposed based on the modeling by quadratic equations. It will provide a guideline for establishing high-yield populations of two-season late Japonica.