A strain of yeast ZJH1 capable of reducing Cr(Ⅵ) was isolated and screened from the intestinal juice of Plodia interpunctella (Hübener) larvae polyethylene plastic. The results of morphological characteristics and molecular biological identification indicated that the strain is Pichia guilliermondii. The minimum inhibitory concentration (MIC) of Cr（Ⅵ） was 17 mmol/L. Under the condition of 50 mg/L Cr（Ⅵ）, the MIC of other heavy metals was Cu（Ⅱ）13 mmol/L,Pb（Ⅱ）6 mmol/L,Ag（Ⅰ）4 mmol/L,Mn（Ⅱ）16 mmol/L,Ni（Ⅱ） 3 mmol/L,Co（Ⅱ） 3 mmol/L,Cd（Ⅱ） 0.9 mmol/L and Hg（Ⅱ） 0.2 mmol/L. The effects of temperature,pH,inoculum size,electron donor and different heavy metal ions on Cr（Ⅵ） reduction rate was investigated by Cr(Ⅵ) reduction rate. The results showed that the optimal pH and temperature of strain ZJH1 for reducing Cr(Ⅵ) were 9.0 and 40 °C in yeast leachate glucose liquid medium (YPD). Under optimal conditions,the inoculum was 8%. When Cr(Ⅵ) is the best,the reduction rate of Cr（Ⅵ） is 100% within 48 h when glucose is used as the electron donor. The strain contains Cu(Ⅱ) in the culture solution containing different heavy metal ions. Except for Pb(Ⅱ) and Co(Ⅱ),both the growth ability and ability of strain ZJH1 to reduce Cr（Ⅵ）were inhibited. The order of inhibition was Mn(Ⅱ)＞Hg(Ⅱ)＞Ag(Ⅰ)＞Ni(Ⅱ) )＞Cd(Ⅱ). The result of a preliminary study on the ability of strain ZJH1 to reduce Cr（Ⅵ） showed that strain ZJH1 has good application potential in the remediation of Cr(Ⅵ) pollution.