In southeast Yunnan typical karst wetland’s black river basin as the research object,under the precondition of regionalization goal set,based on the regional ecological environment survey combined with geographic information system and remote sensing tools,from the importance of the ecological environment sensitivity and ecological service function and advantage of area development degree of three aspects,the importance of the ecological environment sensitivity and ecological service function,regional differentiation rules,the study area is divided into three district six subregion (specific),and based on this division,the black river basin ecological function areas,clear the area of the main ecological problems,points out its developing direction.(1) agricultural and forestry ecological agriculture construction function zone in western China:low agricultural production structure,regional economic development needs to be based on water protection;(2) the eastern ecotourism functional zone:the livelihood mode is not properly matched,and the region should combine the ecological and environmental protection concept with the economic development structure;(3) ecological protection function area of the southern wetland:water conservation and water and soil conservation are prominent,but industrial pollution is serious,so relevant water and soil conservation measures should be taken to control industrial pollution in the region.