As the widely commercial Chinese perch species in China,the unique feeding habits and feeding regulation mechanism of Chinese perch have always been the focus of many scientific researchers. Taking Siniperca chuatsi,a carnivorous fish,as the research object,the peptide YY (PYY) gene was analyzed and identified,and the effect of PYY polypeptide on feeding of Chinese perch was studied by intracerebroventricular (icv) administration. The results showed that the full length cDNA of Chinese perch PYY (sc-PYY) encodes a precursor protein that consists of a putative 28-amino acid signalpeptide,a 36-amino acid mature peptide,an amidation-proteolytic site,and a 30-amino acid carboxy-terminalextension. The sc-PYY gene is comprised of 4 exons interspaced by 3 introns. Amino acid alignment and gene structure comparison indicated that the amino acid sequences of PYY are highly conserved. Among the fishes studied,the consistency of sc-PYY with that of seabass and Japanese pufferfish was higher. Tissue distribution and postprandial changes of the sc-PYY mRNA expression were evaluated by real-time PCR,which showed that the sc-PYY mRNA had the highest expression in the brain,followed by high expression in the intestinal tract,muscles and eyes. The PYY icv results showed that after icv treatment,the food intake of PYY injection group decreased significantly at 4 h compared with control group (PBS). This indicated that PYY might play an important role in the regulation of food intake of Chinese perch.