In this study,the inter-subfamily hybridization of female Megalobrama amblycephala and male Elopichthys bambusa was conducted to obtain the F1 hybrid offspring. Morphological characters of M. amblycephala,E. bambusa and their hybrid offspring were compared and analyzed. The results showed that most morphometric characters of the hybrid fish were closer to those of female parent M. amblycephala. Meristic characters,including the dorsal fin spines,pectoral fin spines,ventral fin spines and anal fin spines of the hybrid fish were consistent with those of both female and male parent. However,for the other characters,such as dorsal fin rays,ventral fin rays,anal fin rays,scale number on lateral line,transversal scales (upper) number,transversal scales (lower) number and swim bladder sacs,the hybrid fish were closer to female parent M. amblycephala than male parent E. bambusa. In addition,we amplified and analyzed the complete mitochondrial genome sequence of M. amblycephala (♀) ×E. bambusa (♂),which is 16 623 bp in length. The overall nucleotide composition was 31.23% A,16.19% G,24.69% T and 27.90% C,with an A+T content of 55.92%. The whole mitogenome contained 13 protein-coding genes,2 rRNA,22 tRNA,and a D-loop region. Phylogenetic analysis showed that M. amblycephala (♀) × E. bambusa (♂) had closer relationship with female parent M. amblycephala than male parent E. bambusa.