To understand the relationship between volatile profiles and ABA content changes in citrus juice sacs,the juice sacs of three pairs of seed/seedless loose-skin mandarin,pummelo and sweet orange were separated,cultured in vitro on medium and treated with 100 μmol/L ABA.After being incubated for 10 d at room temperature,the volatile profiles in the juice sacs were thus detected via gas chromatograph-mass spectrometry.The results showed that ABA treatment had significant dual effects on the contents of monoterpenoids and sesquiterpenoids in the juice sacs of Huanong Red pummelo:δ-elemene,β-cubebene,β-ylangene and β-elemene were significantly higher than those of the control,while the elemene isomer and caryophyllene were significantly reduced; while the contents of sesquiterpenoids such as β-copaene and epicubebol were reduced to non-detectable levels in Newhall navel orange. After ABA treatment,four substances (β-myrcene,γ-terpinene,germacrene D and (2Z,5Z)-2,5-pentadecadiene-1-ol) were newly detected in Guoqing No.1 Satsuma mandarin,whilst D-limonene and β-elemene increased by 10 folds; the contents of β-myrcene and dihydro-3-methylene-2,5-furandione were significantly reduced in seedless Guanximiyou pummelo in ABA treated juice sacs.ABA treatment had no significant effect on volatile profiles in juice sacs of Huanongbendizao mandarin and Early-Gold sweet orange.Collectively,it indicates that ABA treatment did affect the volatile profile in citrus,especially monoterpenoids and sesquiterpenoids,the effect on the aroma quality of the fruit varies with germplasm,but no clear correlation with the existence of seeds or not.