A new technology called sugarfree tissue culture technology was used to increase the quality of potato testtube seedlings and optimize the production,and to improve the technical system of virusfree seed potato breeding. The virusfree testtube seedlings of earlymaturing potato cultivar “Huashu 1” and the traditional MS medium with 4% sucrose was used as the test and control (CK) to compare two different culture methods. The effects of different media,supports and inoculation methods on the growth and development of potato plants in sugarfree tissue culture were studied. The results showed that the testtube seedlings under sugarfree tissue culture grew faster and were more robust,and had more advantages in the production of virusfree potato seedlings compared with the traditional tissue culture. Under sugarfree tissue culture conditions,further removal of organic components from MS medium,only containing inorganic components,had no significant impact on the growth of virusfree seedlings,but more effective in reducing pollution. The results of using vermiculite as support instead of agar showed that the virusfree seedlings supported by vermiculite grew faster,had longer internodes and showed signs of “elongation”. The results under the condition of vermiculite as support culture showed that the seedling growth of doublesegment cutting was significantly better than that of singlesegment cutting. The survival rate of doublesegment cutting was higher,and the segment was larger and easy to operate. The testtube seedlings under sugarfree tissue culture were further cutting into the net room to produce minitubers,of which the number and size had no significant changes compared with that from testtube seedlings under the traditional sugar culture. The new sugarfree tissue culture technology shortens the cultivation period of potato seedlings,reduces the production cost,simplifies the cutting propagation procedure of minituber production. It will lay a foundation for the realization of mechanized cutting of potato seedlings.