Genetic linkage map based on classical molecular marker platforms with low density cannot meet the requirements for wheat functional genomics studies due to its huge and complex genome. 148 recombinant inbred lines (RILs) derived from the crossing Sujata (Indian wheat variety) with Avocet (Australia wheat variety) were used to construct the highdensity genetic linkage map with 6 397 genotypingbyseqencing(GBS) markers,705 DArTarray markers and 164 simple sequence repeat (SSR) markers. 21 chromosomes were covered by 25 linkage groups with a total length of 6 104.4 cM. It will provide an important resource for QTL mapping,mapbased cloning and marker assisted selection in wheat breeding.