Abstract:The aim was to construct a safe and efficient naringin nutrient delivery system. Whey protein isolate (WPI) was used as emulsifier,the preparation conditions were studied for naringin monolayer nanoemulsion (NA/WPΙe) and bilayer nanoemulsion with ιcarrageenan and gum arabic. Subsequently,the stability of the NA/WPΙe and naringin bilayer nanoemulsion was investigated under different environmental factors. The results showed that the optimal preparation conditions of NA/WPΙe were:5 g/100 mL WPI concentration,10% oiltoaqueous ratio(V/V),68.95 MPa homogenization pressure for 3 cycles. The naringin encapsulation ratio could reach 75.50% under this condition.For the bilayers nanoemulsion with 0.3 g/100 mL of gum arabic and ιcarrageenan,naringin encapsulation ratios were the highest,which were 69.80% and 64.78%,respectively. The naringin bilayer nanoemulsion’s droplets were significantly smaller than NA/WPΙe at 100℃ water bath for 10 min and different NaCl concentrations (P<0.05). After 35 days of storage,the growth rate of bilayer nanoemulsion’s droplets was significantly lower than that of NA/WPΙe(P<0.05). And the storage stability increased with increasing concentration of ιcarrageenan and gum arabic. Bilayer nanoemulsion with ιcarrageenan owned higher ionic stability and storage stability than that with gum arabic. The naringin bilayer nanoemulsion improved the stability of NA/WPΙe at its isoelectric point. In conclusion,the bilayer nanoemulsion prepared by adding gum arabic and ιcarrageenan had better physical stability,so it can provide better protection for naringin.