In order to study the regulation mechanism of homeostasis in vivo,Steap4 gene,which was upregulated specifically,was obtained by screening the transcriptome data of highfatfeeding mice.Polymorphism of STEAP4 among different species was compared,and the changes of autophagy related genes at transcription and translation levels were detected 24 hours after transfection in HepG2 cells.The results showed that STEAP4 was conservative among different species,their gene polymorphism mainly changed at the protein level,and the similarity of STEAP4 between mouse and human was the highest.The results of fluorescence quantitative PCR showed that mSTEAP4 had no significant effect on autophagy genes in HepG2 cells,and the results of Western blot showed that mSTEAP4 could reduce the expression of autophagy related proteins,suggesting that mSTEAP4 inhibits the autophagy at the protein level in HepG2 cells.STEAP4 could inhibit autophagy in homeostasis,reduce material and energy metabolism,and keep cells in the resting state.