The candidate genes of degradating imazethapyr were screened with integration analyses of transcriptomics and proteomics data to explore the molecular mechanism of degradating imazethapyr in Trichoderma brevicompactum. The results showed that when the basal medium with 500 mg/kg mazethapyr was used as the sole carbon source to cultivate T. brevicompactum for seven days, the highest degradation efficiency was obtained. There were 1 329 genes with significant transcriptomic differences, of which 703 were upregulated and 626 were downregulated. 21 883 peptides and 4 003 proteins were identified by proteome analyses performed with ITRAQ. The results of association analyses showed that 24 genes with differential expression and protein abundance were correspondingly changed, of which 14 were upregulated and 10 were downregulated. The association coefficient between quantitative protein and gene expression was -0.047 2. The association coefficient between significant difference protein and genes with significantly differential expression was -0.142 0, with the similar trend of expression change. The association coefficient between protein and gene expression was -0.741 3.The association coefficient between protein and genes with the opposite trend of expression change was -0.791 6. Two of the 24 genes identified by association analysis had functional annotations. They are Trichoderma virens Gv298 glycoside hydrolase family 16 protein partial mRNA and HEX1 gene, respectively. They can be used as candidate genes of degradating imazethapyr. It is indicated that the candidate genes of degradating imazethapyr by T. brevicompactum can be effectively screened with the association analyses of transcriptome and proteome.