To clarify the flavonoid glycosyltransferase gene(RsUFGTs) involved in the biosynthesis of anthocyanin in red radish,three candidate RsUFGTs genes were screened from the radish genome,and the open reading frames (ORFs) of three RsUFGTs were cloned and sequenced and expressed. Results showed that the ORF length of RsUFGT1 and RsUFGT2 was 1 383 bp and 1 374 bp,encoding 460 and 457 amino acids,respectively. However,RsUFGT3 was mutated with early termination. Protein sequence alignment and phylogenetic tree analyses indicated that RsUFGT1 and RsUFGT2 both contain PSPG conserved domain and belong to the same UFGT class. The results of realtime quantitative PCR analysis showed that the expression of RsUFGT1 in ‘Hongxin No.1’ was relatively high and no expression was detected in skin of ‘Mantanghong’ and in skin and flesh of ‘Chunbulao’. The expression of RsUFGT1 was positively correlated with anthocyanins content in tissues of different radish cultivars. However,no obvious rules of RsUFGT2 expression were detected. It is indicated that RsUFGT1 may be one of the important structural genes of anthocyanin biosynthesis in red radish.
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杜丽娜,王琪,王春兰,苏睿,谭春艳,蒲燕,陈发波,赖彪. Cloning and expression of RsUFGTs in red radish[J]. Jorunal of Huazhong Agricultural University,2019,38(4).