In order to understand the clinical antimicrobial resistance of Staphylococci and their multidrug resistance gene cfr from animals in Xinjiang,and to provide guidance for clinical medication and further study of the cfr gene transmission mechanism,a total of 1 781 animal feces and nasal samples were collected from some areas of Xinjiang Uygur Autonomous Region.A total of 1 667 strains of Staphylococci were isolated from the samples,and then drug sensitivity tests of 11 kinds of clinical antibiotics were carried out.The PCR method was used to detect the amide-alcohol resistance genes ( cfr , fexA ,and fexB ),the mobile elements IS 21-558 and transposase gene Tn 558 ,which were related to the transfer of cfr .The results showed that the order of severity of drug resistance of Staphylococci to clinical commonly used antimicrobial agents was sheep source＞pet source＞pig source＞poultry source＞cattle source. The penicillin resistance was the most serious among the tested antibiotics,and the drug resistance rate was between 54.7% and 93.6%. Except for Staphylococci isolated from cattle (36.3%),the resistance rate of Staphylococci isolated from other animals to oxacillin was 51.1%-98.2%. Staphylococcus from animals showed good sensitivity to amikacin,gentamicin and clindamycin. Among the 11 clinical antibiotics,the multidrug resistance rate of Staphylococcus was concentrated in 4-5 from the sheep source,was concentrated in 3-5 from the poultry and pet source,was mainly 4 and 7 from the pig source, and was 1 from the bovine source. Staphylococcus isolated from different animal sources had a wide range of antibiotics resistance spectrum. The detection results of amide-alcohol resistance gene showed that,only 4 strains of Staphylococcus isolated from pigs carried cfr gene. Except for bovine source Staphylococcus , fexA gene was detected in other animal sources with the order of detection rate of pet source＞pig source＞poultry source＞sheep source,but the fexB gene was not detected. The 16S rRNA sequence amplification results showed that the 4 strains carrying the cfr gene were all Staphylococcus simulans . In addition,mobile element IS 21-558 (including istAS and istBS ) and Tn 558 transposase gene ( tnpA , tnpB , tnpC ) were detected in 4 positive bacteria. From above,the resistance of Staphylococcus from animal origin was serious in Xinjiang,the spectrum of resistance was diverse,and the main gene of amide-alcohol resistance was the fexA gene. In addition,multidrug resistance gene cfr and its mobile components were detected. It is suggested that the surveillance of drug resistance should be strengthened to prevent the outbreak of drug resistance bacteria and the probable emergence of super resistant bacteria.