Based on the previous droughtassociatedgeneenriched SSHcDNA library and cDNAmicroarray,we preliminarily screened an HuABAR Unigene presumably involving in the tolerance of abiotic stresses including drought,cold and high temperature.Fulllength cDNA sequence of this gene was cloned.Bioinformatic analyses and subcellular localization was carried out.The results showed that HuABAR gene was significantly upregulated in response to abiotic stresses including in drought,high temperature and low temperature.The highest expression level was observed at the 5th day or the third day after drought stress or high temperature stress.The expression was increasingly unregulated as exposure to low temperature within five days.HuABAR gene contained 1 239 bp in full cDNA length,and consisted of 264 bp 5′UTR,414 bp 3′UTR,and 561 bp open reading frame (ORF) encoding 187 amino acids.This gene might encode a typical SRPBCC domain,and was highly similar to the PYR1/PYLs (pyrabactin resistance 1/PYR1 like) family.The target gene was ligated with green fluorescent protein (GFP) by constructing plant transient expression vector pBWA (V) HSHuABARgfp,and the PEGmediated method was used to transiently transform into Arabidopsis protoplasts.It was found that target gene was located in the cytoplasm,which satisfied the expectations.It is indicated that HuABAR in pitaya may be involved tolerance of multiple abiotic stresses.