The iridophores in the fish skin could not be observed by traditional paraffin section method,so Paramisgurnus dabryanus was used to improve the method in this experiment.The fixative was changed to 10% neutral formalin.Moreover,1.5％ agarose was used to pretreat the skin before dehydration,and 3％ eosin was used to stain the skin.The results showed that fixing the skin of P. dabryanus by 10％ formalin,pretreating by 1.5％ agarose before dehydration and staining with 3％ eosin,could get the slices with intact organizational structure and clear iridophores.Therefore,the improved paraffin section method could be used to observe iridophores in the fish skin.