MYB transcription factors play an important role in the response under low phosphorus stress.To further confirm the role of PmMYB169 cloned from masson pine (Pinus massoniana) in the response under low phosphorus stress,the pBWA(V)HS-PmMYB169-GLosgfp vector was constructed and subcellular localization of PmMYB169 was comducted in tobacco. Then,PmMYB169 was transformed into tobacco via Agrobacterium-mediated method,and the tolerances of over-expressed-PmMYB169 transgenic lines to low phosphorus stress were analyzed. The results showed that the PmMYB169 had function in the nucleus,and 22 transgenic lines were obtained.The phosphorus contents,biomass and activities of Apase,POD and SOD were significantly higher than those of the wild type under low phosphorus stress. There is no significant differences among the normal and higher phosphorus treatments. It is indicated that over-expression of PmMYB169 significantly increase the response ability of plants under low phosphorus stress.