The promoter regions of ZmCBL1 and ZmCBL9 were cloned to study the differences in expression,regulation,and function of ZmCBL1 and ZmCBL9 in maize.Results of PlantCARE analyses showed that the two promoters contained multiple cis-regulatory elements in response to drought,light,and ABA.Results of GUS activity analyses showed that ZmCBL1 and ZmCBL9 were not induced by cold stress but induced by mannitol,darkness and ABA,but their expression levels were slightly different.Due to the different regulatory elements in their sequences,there is a difference in their response to GA3 and wound stress.In addition to differences in expression under stress-induced conditions,the expression of these two promoters differed in organs (tissue) and developmental stages.Only the ZmCBL1 promoter was expressed in filaments and stigmas.It is indicated that there may be some redundancy in the functions of ZmCBL1 and ZmCBL9 at different stages of stress and development,but there are significant differences as well.