An experiment was conducted for 30 d to investigate the effect of Bacillus subtilis TL on the growth development,intestinal environment and health status of weaned piglets. A total of 120 Duroc×Landrace×Yorkshire pigs were randomly divided into 4 groups,the negative control group (basal diet),positive control group (basal diet +2.5 g/kg antibiotic),M1 group (positive control group+150 g/t antibiotics) and M2 group (positive control group +300 g/t antibiotics). The results showed that the weight,average daily gain and the average daily feed intake in the M1 group increased by 7.81% (P<0.05),17.14% (P<0.01),and 33.10% (P<0.01),respectively,compared with the positive control in terms of growth performance. In addition,the diarrhea rate of weaned piglets in the M1 and M2 groups were significantly reduced. The serum GSH-Px,T-SOD and the swine fever antibody were increased by 9.0% (P<0.05),32.78% (P<0.05) and 14.06% (P<0.01),respectively,while the MDA was decreased by 43.71% (P<0.05),compared with the positive control in terms of blood index. Moreover,the small intestinal villus was significantly increased and the depth of crypt was significantly decreased compared with the positive control in terms of intestinal barrier. At the same time,the number of cecum Lactobacillus in the M1 group was significantly increased and the number of cecum E. coli were significantly reduced,compared with the positive and negative control. It was concluded that B. subtilis TL could significantly improve the growth performance,the intestinal environment and health status of weaned piglets.