To identify the function of CAZymes proteins in Colletotrichum higginsianum, the physical and chemical properties were analyzed based on the 238 CAZymes proteins sequence. The results showed that the protein isoelectric point was less than 6 in nearly two-thirds proteins,belonging to acidic protein. Ten percent of the sample proteins were then screened by the random number software,to define the molecular mass,the molecular formula,half-life,the instability factor,aliphatic amino acid index,and total average hydrophilicity properties of secreted protein from different theoretical isoelectric point category.