“Huangyou No.1” (Pelteobagrus vachelli♂×Pelteobagrus fulvidraco♀) is a new freshwater fish breed with advantages of rapid growth and strong resistance to disease.To appraise the nutritional value of “Huangyou No.1”,we analyzed the composition and content of amino acids and fatty acids in the muscle of “Huangyou No.1” and compared it with that of its parental species.A total of 17 amino acids were detected in the muscle of the three kinds yellow catfish.The contents of the total amino acids,total essential amino acids and total flavor amino acids were highest in the muscle of “Huangyou No.1” among the three kinds of yellow catfish.The content of essential fatty acids in the muscle of “Huangyou No.1” was 22.85%,which was close to that of yellow catfish and slightly lower than that of darkbarbel catfish.These results showed that “Huangyou No.1” has significant nutritional advantages in fatty acids and amino acids.