To elucidate the expression pattern and effect of temperature stress of thioredoxin peroxidase inHeortia vitessoides (HvTpx),the cDNA sequence of HvTpx was screened from the transcriptome of the adult H. vitessoides. The complete open reading frame (ORF) of HvTpx is 588 bp in length,encoding 195 amino acids. HvTpx is a typical member of the 2-Cys Tpxs family,which showed the highest similarity with Helicoverpa armigera(87%) and shared similarity above 75% with other insects.H. vitessoides and H. armigera were clustered in the same branch in the phylogenetic tree based on insect thioredoxin peroxidase amino acids. The results of RT-qPCR revealed that the expression levels of HvTpx were higher in adult than in other stages during development. In larvae,HvTpxhad the highest expression level in midgut. HvTpx showed the highest expression level in the abdomen and lowest in the foot in adults. The expression levels of HvTpxat 0℃,10℃ and 35℃ were significantly increased than that in the control (25℃),indicating that expression of HvTpx could be induced by temperature. The results provide further information in understanding the molecular mechanism of H. vitessoides response to temperature stress.