In a previous study,six autophagy-related genes were identified during sclerotial development of Rhizoctonia solani AG1-IA using RNA-seq technology.In order to reveal the expression patterns of these genes during sclerotial development of R. solani AG1-IA ,quantitative real-time PCR (qRT-PCR) was performed.The results showed that expression of the serine/threonine kinase，ssp1, ATG13 and Vam6 genes was significantly up-regulated at the sclerotial initiation stage,but down-regulated at the sclerotial maturation stage.However,the serine/threonine-protein phosphatase gene and serine/threonine kinase receptor-related protein gene showed a significant decrease during sclerotial development of R. solani AGI-IA.The expression patterns of these genes obtained by qRT-PCR were in accordance with the results obtained by RNA-seq,which indicated that the results of RNA-seq were reliable.This study also showed that autophagy-related genes were highly regulated during sclerotial development,and may play an important role in sclerotial development.