Construction and expression analyses of nodule symbiosis genes route inArabidopsis thaliana
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Abstract:
Ten key genes in symbiosis signaling from Lotus japonicuswere controlled under ten different individual promoters expressed in roots of Arabidopsis thalianawith biotechnology and synthetic biology approaches.Two multigenes vectors,pUNS6 containing 6 genes and pDNS4 containing 4 genes,were constructed respectively to transform Arabidopsis thaliana.The result of complement assay showed that ten genes controlled in CaMV35S promoter were able to restore symbiotic function of relative genes mutant in leguminous plants with hairy root transformation.The pUNS6 and pDNS4 were transformed into Arabidopsis with Agrobacterium tumefaciens-mediated inflorescence infection respectively.The result of identifying the transgenic T0,T1 and T2 plants by PCR and RT-PCR showed that those of foreign symbiotic genes were stably existed on genome and were able to express at the RNA level in Arabidopsis thaliana.
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梅江鹏,李豪,张忠明. Construction and expression analyses of nodule symbiosis genes route inArabidopsis thaliana[J]. Jorunal of Huazhong Agricultural University,2018,37(2):28-33.