Ten key genes in symbiosis signaling from Lotus japonicuswere controlled under ten different individual promoters expressed in roots of Arabidopsis thalianawith biotechnology and synthetic biology approaches.Two multigenes vectors，pUNS6 containing 6 genes and pDNS4 containing 4 genes,were constructed respectively to transform Arabidopsis thaliana.The result of complement assay showed that ten genes controlled in CaMV35S promoter were able to restore symbiotic function of relative genes mutant in leguminous plants with hairy root transformation.The pUNS6 and pDNS4 were transformed into Arabidopsis with Agrobacterium tumefaciens-mediated inflorescence infection respectively.The result of identifying the transgenic T0,T1 and T2 plants by PCR and RT-PCR showed that those of foreign symbiotic genes were stably existed on genome and were able to express at the RNA level in Arabidopsis thaliana.