Somatic embryogenesis receptor protein kinase gene (SERKs) belongs to the family of genes encoding LRR receptor kinase and participates in multiple life activities including plant growth,development and immunity.Eight homologous genes (LjSERKs) were identified from Arabidopsis thaliana AtSERKs gene sequence to explore the function and mechanism of SERKs gene family in the process of nodule symbiosis.Using the CRISPR-Cas9 genome editing and hair root transformation technique,the different genes of the LjSERKs family were knocked out separately.The effects on the symbiotic nodule phenotype were observed and analyzed.The results showed that the number of nodules was not different significantly from that of the control plants after knockouting LjSERK2,LjSERK3,LjSERK6 and LjSERK7.The number of nodules was significantly decreased after knockouting LjSERK8.It is indicated that LjSERK8 is involved in the nodule symbiosis process and there may be functional redundancy between other genes in the family.