There are two fertility restorer genes,Rf5 and Rf6 in 9311 and two sterility genes orfH79 and orf216 in YTA.To study the interaction between two restorer genes and two CMS genes in Honglian (HL) rice,HL-CMS line YTA was crossed with the restore line 9311.Then,F1 was backcrossed repeatedly as the male parent with the YTA as recurrent male parent for more than eight generations.Based on the sequences of Rf5 ,we designed the specific primers and tested the restorer gene in L-Rf5.Combing with the pollen fertility,we did not find genetic divergence in self-crossed progenies.It suggested that the homozygous L-Rf5 was constructed successfully.By analyzing the expression level of HL-CMS gene orfH79 and orf216 in L-Rf5,we found that the expression level of orfH79 in L-Rf5 decreased distinctly than that in YTA.There is no obvious difference between the homozygous L-Rf5 and the heterozygous L-Rf5.However，The expression of orf216 in L-Rf5 was not significantly decreased compared with YTA.Meanwhile,the Rf1B sequences of YTA,9311,near isogenic lines were cloned,and we found no difference in these materials.This study laid the foundation for molecular analysis of the restoration of fertility gene Rf1B.