The physical and chemical properties and bioactivities of the two kinds of SDF from black and white glutinous rice bran were studied to explore the possible role of SDF in lowering the blood glucose levels after the meal by digesting and absorbing glucose in vitro.The results showed that two samples had the typical characteristic of polysaccharide based on analyzing the infrared and ultraviolet spectrum.The contents of phenol,viscosity,molecular weight,assembly of aggregated particles,porosity of surface and pore size,oxidation resistance of SDF from black glutinous rice bran was favorable for absorbing glucose and inhibiting glucose diffusion,better than that of SDF from white glutinous rice bran.But the dissolution rate,the ability of inhibiting starch digestion and α-amylase activity of SDF from white glutinous rice bran was higher than that of SDF from black glutinous rice bran.It is indicated that the nutritional value and bioactivity of SDF from black glutinous rice bran is better than that from white glutinous rice bran.