The full-length coding sequence of the nueleoprotein (N) gene in spring virema of carp virus（SVCV） was amplified by PCR method using N-RFP plasmid as the template. The PCR production was cloned into the prokaryotic expression vector pGEX-KG. The fusion protein SVCV-N-KG was analyzed by SDS-PAGE. The BALB/c mice was immuned by purified SVCV-N-KG protein and the serum titer was determined by ELISA. One monoclonal antibody (McAb N-2) against N protein were generated and selected by ELISA and chromosome number analysis after four times of subcloning selection. McAb N-2 was characterized by IFA (indirect immunofluorescent assay) and Western blot assay. Further analysis showed that McAb N-2 targeted the 227-336 amino acid region of N protein. This study laid a foundation for the study of pathological mechanisms and clinical diagnosis of SVCV.