The majority of carbohydrate impurities in the mixture of α-arbutin enzymatic synthesis reaction were removed with alcoholic fermentation.An activated carbon fiber column was used to enrich α-arbutin with water and different concentration ethanol elution.The final recrystallization with methanol-ethyl acetate mixed solvent was used to obtain α-arbutin crystal.The results showed that the 50% ethanol solution elution part had the highest concentration of α-arbutin.After concentrated by vacuum concentration,this part was recrystallized with methanol-ethyl acetate mixture.Some colorless,needle-like α-arbutin crystal was obtained.The purity of crystal was over 99% α-arbutin by HPLC analysis.The established method is useful for industry production with its simplicity,low cost and high purified product.It will provide reliable parameters for the massive industry production.