To investigate the impact of biochar on Prunus pseudocerasu soil microbial and nutrients property,one of the P. pseudocerasu varieties ‘Manaohong’ cultivated in Guizhou was used as materials,aiming at improve the production and quality of the P. pseudocerasu and provide a theoretical basis for the application of biochar on fruit.Test was set up with the three different biochar application rates,including 0 (CK),5 (C1) and 10 kg (C2) pet plant were applied in the protected field.Results showed that at the initial blossoming stage,C1 treatment could effectively increase the quantity of bacteria,denitrifying bacteria and C2 increase the quantity of azotobacter significantly; in the full-bloom stage,the treatment of C1 improved the quantity of denitrifying bacteria and the quantity of azotobacter,denitrifying bacteria were significantly increased under the C2 treatment.Meanwhile,the microbial diversity on soil with the treatment of C1 and C2 were significantly higher than CK; on the fruiting stage,the quantity of fungi was effectively increased under the C2 treatment; applying biochar increased the soil pH and the content of organic matter,but the impact on soil nutrients varied with the phenological period.Our research indicated that applying biochar on soil could increase the amount phenophase and diversity of microorganisms of P. pseudocerasu ‘Manaohong’, as well as soil nutrients,while the raising efficiency was close related to the growth status of plants.