To identify the differences among physiological races of Fusarium oxysporum f. sp. cubense (Foc),the cell wall-degrading enzymes,esterase isozyme and soluble protein of this pathogen were tested and analyzed. The results showed that four kinds of cell wall-degrading enzymes including polygalacturonase (PG),pectin methyl-galacturonase (PMG),celluloses (Cx) and filter paper enzyme (FPA) were detected in all the four physiological races of Foc from domestic and abroad. The PG and Cx activities of Foc 4 were the highest. The Cx activities of Foc 1 and Foc 4 from abroad were higher than those of the same race from domestic. The FPA activity of Foc 1 from domestic was the lowest,while there was no significant difference among other races. The PG activity showed no significant difference in the same race from domestic and abroad. The electrophoretograms of esterase isozyme and soluble protein indicted that great difference existed among all the four races. Cluster analysis further demonstrated that the isolates with similar pathogenicity were clustered into the same branch,which had close genetic relationship.