Cotton cultivars including E′kangmian-10 and Tongza 411 were used to study the mechanical damage of cotton bowl seedling during seedling transplanting.The shear and bending of cotton seedling was tested at different loading rate.The cotton bowl was tested with compression.Twofactor variance analysis with duplication was used to analyze shear strength and bending strength.The results showed that the shear strength and bending strength was increased with the increase of the loading rates.When loading rate was the same,the shear strength and bending strength of E′kangmian 10 were greater than that of Tongza 411.Both the load rate and varieties had significant effect on the shear strength and bending strength.The interaction between them had no significant effect on the shear strength and bending strength of cotton seedling.Bowl rupture force was 73.3-110.5 N and 100.3-192.3 N at radial and axial compression.The anti-extrusion capability of bowl had anisotropies.It will provide a theoretical basis for the design of mechanically transplanting the cotton seedling.