Serum from 2 063 pigs from a slaughterhouse in Liaoning Province were collected and detected by modified agglutination test (MAT).The results showed that the samples with titer≥25 were 233 and the positive rate was 11.3%.A total of 67 pigs with titer≥100 were chosen for Toxoplasma gondii isolation,in which 23 T. gondii strains were isolated from the heart tissues with an isolation rate of 34.3%.In this study,T. gondii strains were isolated successfully,showing the infectious status of pig toxoplasmosis in Liaoning Province,which poses a huge potential threat to public health.