Trichoderma reesei QM6a was an important industrial production strains for cellulase production.To identify the secreted protein from T. reesei and clear its characteristic,9 143 protein sequences in T. reesei was analyzed to predict the secreted protein using 5 programs including SignalP,ProtComp,TMHMM,bigPI Fungal predictor and TargetP. Meanwhile,the distribution of amino acids,the length of signal peptide as well as the signal peptide cleavage site of secreted protein were analyzed.A total of 356 secrete proteins were found in T. reesei,and the length of amino acids and the signal peptide were different from the plant pathogens.The signal peptide cleavage site belonged to AXA type,which was consistent with other plant pathogenic fungi,bacteria and oomycete.Through the above bioinformatics analysis,the prediction of secreted proteins can be effectively achieved in T. reesei,and the type of signal peptide cleavage site was consistent with other secreted proteins from different species.