To accurately identify Fusarium wilt, an important pathogen of asparagus, and to make its taxonomic status clear, the identification was carried out using morphology examination and BLASTn based on rDNA ITS sequences, the differences of rDNA ITS sequences between Fusarium wilt fungus of asparagus and its related fungi were compared, and the phylogenetic relationship was analyzed. The result revealed that the Fusarium wilt fungus of asparagus was Fusarium oxysporum f. sp. asparagi. The differences of rDNA ITS sequences were at 76-80 bp, 104-115 bp, 129-138 bp, 151-158 bp, 175-178 bp, 382-402 bp, 438-445 bp and 473-479 bp regions between the Fusarium wilt fungus of asparagus and its related fungi from the same genus. Fungi from the 6 genera were clustered as 2 groups and 2 subgroups, and F. oxysporum f. sp. asparagi was phylogeneticly nearest related with F. equiseti and F. incarnatum.