The relationships between autophagy and senesence in rice under darkness condition were discussed. The phenotype of OsATG7 mutants was observed. OsATG7 transgenic plants were overexpressed and interfered. The expression of OsATG7, SGR gene and the variation of chlorophyll content in different genotypes of OsATG7 mutants treated with darkness was studied. Results showed that the expression of OsATG7 was associated with rice fertility. The expression of OsATG7 and SGR gene increased sharply in homozygous mutant. The chlorophyll content of OSATG7 mutants decreased faster than that of control plants. Under darkness condition, the autophagosome only formed in control plants rather than homozygous mutants.