In order to obtain more microorganism resources to control soybean cyst nematodes (SCN),control efficiency of three fungal strains including Aspergillus niger NBC001, Penicillium oxalicum NBC008 and P. oxalicum NBC012 on soybean cyst nematodes were evaluated. In vivo analyses showed that all three fungi fermentation were lethal to nematode juveniles. The revised mortality of 2 times dilutions against SCN juveniles was about 90%,and the inhibition of egg hatchability was>68%. The pot experiment showed that cyst number treated with NBC001,NBC008,NBC012 fermentations were reduced by 50.07%,59.88%,57.45%,respectively,compared with the control . The height of plant increased by 11.3% and 18.6% after treated with NBC001 and NBC012 fermentations,while the length of root increased by 26.7% after treated with NBC008 fermentations. The present investigation suggested that fermentations of NBC001,NBC008,NBC012 could be used to control soybean cyst nematode.