LC-MS based metabolomics analysis was used to explore metabolic profile of Coniothyrium minitans co-culture with its host fungus Sclerotinia sclerotiorumThe results showed that coculture could significantly influence fungal metabolic profile.Three metabolites,namely Macrosphelide A,Benzenediol,and 5Aminopentanoate,were identified in C. minitans specific metabolites database and the largest metabolites accumulation was detected at 2 d of coculture.This approach allowed the investigation of metabolic patterns in mycoparasitism and indicated that mycoparasitism could induce metabolites communication and secondary metabolites accumulation.