Based on the continuous measurement of the soil respiration of typical Pinus massoniana forest in Zigui County of Three Gorges Reservoir Area,the spatial variation characteristics of soil respiration in sample scale was studied. The effects of factors including physical and chemical properties of soil,microbial biomass and fine root biomass on spatial heterogeneity of soil respiration were analyzed using geostatistics combining with the classic statistics. The results showed that soil respiration had an obvious seasonal dynamics due to the changes of soil temperature among different seasons. Soil respiration had a moderate variation in each season with a coefficient of variation between 32.6% and 46.3%. Soil respiration had a spatial autocorrelation characteristic and a clear patchy distribution,and could be described well by spherical model or exponential model. Spatial variation of soil respiration in the forest stand was controlled by the biomass of fine root,the porosity of the surface layer soil and the soil available nitrogen.