To study the Cucumber mosaic virus (CMV) transmission pattern and symptoms,quantitative real-time PCR and RT-PCR assay and visual inspection were performed in differentiated banana shoots of different varieties infected by CMV. The results showed that there were no obvious symptoms of the differentiated banana shoots in different varieties such as ‘Yueyoukang No.1’,‘Williams’,‘Dajiao’ and ‘Huangdijiao’. The concentrations of CMV in the differentiated banana shoots of these varieties were not the same in different generations. However,the CMV concentrations of the top-one or (and) top-two leaves were higher than those in other leaves. Hence,the top leaves of differentiated banana shoots could be collected as samples for virus detection. 