In order to apply the technology of in vitro lamb embryo development to production,we studied the possible effects of factors such as dose of FSH,individual rams,oocyte obtain methods on in vitro embryo development and the conception efficiency after transplantation. The results showed that injection dose of FSH 240 IU or 420 IU per donor had no effect on the number of obtained oocytes,the number of embryos,and in vitro fertilization and cleavage rate (P＞0.05). Ram had significant effects on in vitro oocyte fertilization efficiency (P< 0.05),but did not affect the pregnancy rate of embryo transplantation (P＞0.05). When compare oocyte harvesting method of ovum pick up (OPU) with slaughterhouse,there was no effect on the recovery of oocyte (12.24 vs. 13.84,P>0.05). However,the average number of embryos obtained by OPU was significantly lower than that obtained by slaughterhouse (7.50 vs 11.17,P<0.05),and the cleavage rate was extremely significantly lower than the latter (60.92% vs 81.75%,P<0.01). All of the above tests had no effect on the pregnancy rate (P＞0.05). Using 240 IU donor FSH and selecting the fertilization efficiency ram,can significantly improve the in vitro embryo production. The in vitro developmental ability of the oocyte harvested by the slaughterhouse was higher than that by OPU.