Effects of 4 common preservation methods (5% formalin,10% formalin,95% alcohol and -20℃ frozen) on spore morphology of Myxobolus wulii were investigated in the present study.Compared with fresh spores,only the spores preserved in 5% formalin for 7 d showed no significant differences in measurements (P<0.05),which indicated that 5% formalin is a suitable fixative for M.wulii spores preservation for a short period of time (less than 7 d).The change rates of measurement indexes of preserved spores were different,indicating that the present preservation methods made M.wulii spores deform irregularly.For spores preserved by different preservation methods in the same time period,95% alcohol yielded the maximum contraction,following by -20℃ frozen,10% formalin and 5% formalin.The total change rates of spores,except for that preserved in 10% formalin,decreased with the extension of preservation time,indicating that the preserving spores doesn’t always shrink continuously.