A feeding trial was conducted to investigate effects of fish meal (FM),soybean concentrate (SC),canola meal (CM) and a combination of these protein sources at ratio 1∶1∶1 (Mix) on growth performance,whole amino acid compositions and antioxidant ability of loach,Misgurnus anguillillcaudatus fingerling.A total of 1 200 loach with an average initial weight of 35.0± 0.1 mg were fed on the test diets for 30 d in a flow-through fresh water system.The results showed that the body weight gain (BWG) of FM and Mix groups was significantly higher than those of SC and CM groups.Moreover,feed intake (FI) of the fingerling in FM was higher than that of the plant protein groups.Whole body lysine and valine concentrations in essential amino acid of the fingerling fed on CM were significantly lower than those fed on FM.In the term of antioxidant enzyme activities,the fingerling in SC group had higher glutathione peroxidase activity and lower superoxide dismutase activity compared with loach in the FM,CM and Mix diets groups.In conclusion,fish meal is suitable protein source for loach fingerling.